Anti-Mouse TNFR2 (TR75-54.7) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Tumor necrosis factor receptor II (TNF-RII) is present on most cell types and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells

Product Name Alternative

Tnfrsf1b, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b, Tnfr-2

UniProt

P25119

Host

Armenian Hamster

Reactivity

Mouse

Immunogen

Purified Recombinant Mouse TNF R2

Target

TNFR2

Isotype

IgG

Clone

TR75-54.7

Applications

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, ELISA

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

References & Citations

Uncoupling immune trajectories of response and adverse events from anti-PD-1 immunotherapy in hepatocellular carcinoma

Shipping Conditions

Blue ice

Storage Conditions

Anti-TNFR2 In Vivo Antibody - Low Endotoxin (TR75-54.7) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-TNFR2 In Vivo Antibody - Low Endotoxin (TR75-54.7) recognizes Mouse CD120

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Blocking: Clone TR75-54.7 antibody has been reported to block ligand-induced receptor signaling. Each investigator should determine their own optimal working dilution for specific applications.