Anti-Mouse TNF Alpha (TN3-19.12) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity. It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites. TNF-alpha is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-alpha secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-alpha has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha

Product Name Alternative

Cachectin, TNFa, Tumor necrosis factor ligand superfamily member 2, Tnfsf2

UniProt

P06804

Host

Armenian Hamster

Reactivity

Mouse

Immunogen

Recombinant mouse TNFalpha

Target

TNF alpha

Isotype

IgG

Clone

TN3-19.12

Applications

Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, Blocking

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-TNF alpha In Vivo Antibody - Ultra Low Endotoxin [TN3-19.12 ] (ICH1136UL)

Specificity

Anti-TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12) recognizes Mouse TNFa

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)

Antibodies Against Same Target

Anti-TNF alpha In Vivo Antibody - Low Endotoxin [MP6-XT22] (ICH1127)

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