Anti-Mouse Ly-6G/Ly-6C (RB6-8C5) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI) -linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

Product Name Alternative

Ly-6G/LY-6C, Gr-1, Myeloid

Host

Rat

Reactivity

Mouse

Immunogen

Raised against granulocytes of mouse origin

Target

Ly-6G/Ly-6C

Isotype

IgG2b

Clone

RB6-8C5

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assays, IHC (Frozen), IHC (Paraffin), Immunocytochemistry, Immunofluorescence, In Situ Hybridization

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

0.5 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin (RB6-8C5) is stable for at least four (4) week when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin [RB6-8C5] (ICH1131UL)

Specificity

Rat Anti-Mouse Granulocytes (Clone RB6-8C5) recognizes Mouse Granulocytes

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

Antibodies Against Same Target

Anti-Ly-6C In Vivo Antibody - Low Endotoxin [HK1.4] (ICH1111) Anti-Ly-6C In Vivo Antibody - Low Endotoxin [7B10] (ICH1095) Anti-Ly-6G In Vivo Antibody - Low Endotoxin [1A8] (ICH1089) Anti-Ly-6G In Vivo Antibody - Ultra Low Endotoxin [1A8] (ICH1089)