Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.

Product Name Alternative

Leukocyte common antigen, L-CA, T200, CD45, Receptor-type tyrosine-protein phosphatase C, PTPRC, B220

UniProt

P08575

Host

Rat

Reactivity

Mouse, Human

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Target

CD45R

Isotype

IgG2a

Clone

RA3-6B2

Applications

Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

0.5 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) is stable for at least four (4) weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CD45R In Vivo Antibody - Ultra Low Endotoxin [RA3-6B2] (ICH1130UL)

Specificity

Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .

Aggregation

Aggregation level ≤ 5%

Applications Notes

Functional Assay: In vivo treatment with Rat Anti-Mouse CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Antibodies Against Same Target

Anti-CD45 In Vivo Antibody - Low Endotoxin [I3/2.3] (ICH1057)

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