Anti-Mouse NK1.1 (PK136) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Name Alternative

Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c

UniProt

P27814

Host

Mouse

Reactivity

Mouse

Immunogen

NK-1+ cells from mouse spleen and bone marrow

Target

NK1.1

Isotype

IgG2a κ

Clone

PK136

Applications

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, CyTOF, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin [PK136] (ICH1128UL)

Specificity

Anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative using PK136.