Anti-Mouse GM-CSF (MP1-22E9) In Vivo Antibody - Low Endotoxin
Product Specifications
Background
Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.
Product Name Alternative
Csf2, Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, CSF, Csfgm
UniProt
P01587
Host
Rat
Reactivity
Mouse
Immunogen
Yeast Derived Recombinant Mouse GM-CSF
Target
GM-CSF
Isotype
IgG2a
Clone
MP1-22E9
Applications
Western Blot, ELISA, ELISPOT, Blocking, Immunocytochemistry, IHC (Frozen)
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
1.0 - 5.0 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100ml ICH3002-100ml ICH3003-100ml
Shipping Conditions
Blue ice
Storage Conditions
Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) is lyophilized and can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Related Products
Anti-GM-CSF In Vivo Antibody - Ultra Low Endotoxin [MP1-22E9] (ICH1125UL)
Specificity
Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) recognizes an epitope on Mouse GM-CSF
Formulation
0.2 μm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Western Blotting: To detect Mouse GM-CSF this monoclonal antibody can be used at a concentration of 1-2 μg/ml.Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
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