Anti-Mouse GM-CSF (MP1-22E9) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.

Product Name Alternative

Csf2, Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, CSF, Csfgm

UniProt

P01587

Host

Rat

Reactivity

Mouse

Immunogen

Yeast Derived Recombinant Mouse GM-CSF

Target

GM-CSF

Isotype

IgG2a

Clone

MP1-22E9

Applications

Western Blot, ELISA, ELISPOT, Blocking, Immunocytochemistry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) is lyophilized and can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Related Products

Anti-GM-CSF In Vivo Antibody - Ultra Low Endotoxin [MP1-22E9] (ICH1125UL)

Specificity

Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) recognizes an epitope on Mouse GM-CSF

Formulation

0.2 μm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Western Blotting: To detect Mouse GM-CSF this monoclonal antibody can be used at a concentration of 1-2 μg/ml.Each investigator should determine their own optimal working dilution for specific applications.