Anti-Mouse IFNAR1 (MAR1-5A3) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

The antibody when prepared specifically for In vivo functional assays blocks type I IFN receptor signaling both In vitro and In vivo without depleting IFNAR1 bearing cells. This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1. IFNAR1 and IFNAR2 are coexpressed on nearly all cells and make up the heterodimeric receptor that binds all type I IFNs (IFN alpha and beta) . Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.

Product Name Alternative

Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Type I interferon receptor 1, Ifar, Ifnar

UniProt

P33896

Host

Mouse

Reactivity

Mouse

Immunogen

This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.

Target

IFNAR1

Isotype

IgG1

Clone

MAR1-5A3

Applications

Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, ELISA

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. NOTE: Do not freeze as this antibody at -20°C as this clone will sometimes precipitate over long period of time.

Related Products

Anti-IFNAR1 In Vivo Antibody - Ultra Low Endotoxin [MAR1-5A3] (ICH1122UL)

Specificity

Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) recognizes the extracellular domain of the IFNAR1 subunit of the mouse IFN-alpha / beta receptor.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

IFNAR1 and IFNAR2 are coexpressed on nearly all cells.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Blocking: Clone MAR1-5A3 has a short half-life, basically because every cell expresses the IFNAR1 receptor and the receptor recycles very rapidly. And, if you want to block function in vivo, you need to be sure that all of the receptors are blocked continually in all compartments. Therefore, you need a large in vivo loading dose (2.5 mg/mouse) to saturate all the binding sites in vivo and then maintain a high enough level to keep them saturated. For in vivo blocking studies we recommend give a loading dose of 2.5 mg/mouse and follow with a weekly dose of 0.5 mg/mouse. The half-live following a 2.5 mg loading dose is about 5 days. [However, if you only inject a low dose of 250 micrograms, then the half life is 1.5 days – because you haven’t saturated the mouse]. Each investigator should determine their own optimal working dilution for specific applications.