Anti-Mouse IL-1R (JAMA-147) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

IL-1 binds two receptors, IL-1RI and IL-1RII. The ligands IL-1alpha and IL-1beta exert all of their various biological actions by binding IL-1RI. IL-1RII has a shortened cytoplasmic domain that is unable to transduce signals and therefore acts as a decoy receptor and competitively antagonizes the binding of IL-1alpha and IL-1beta, thereby reducing their biological effects. Binding of the ligands to IL-1RI is enhanced by an accessory protein, IL-1R-AcP. IL-1RI is expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. It is an 80 kD transmembrane glycoprotein.

Product Name Alternative

Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, IL-1R-alpha, Interleukin-1 receptor type I, p80, CD121a

UniProt

P13504

Host

Armenian Hamster

Reactivity

Mouse

Immunogen

Extracellular domain of mouse IL-1 R type 1

Target

IL1R1

Isotype

IgG

Clone

JAMA-147

Applications

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-IL-1R In Vivo Antibody - Low Endotoxin (JAMA-147) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles