Anti-Mouse Ly-6C (HK1.4) In Vivo Antibody - Low Endotoxin

Product Specifications

Product Name Alternative

Lymphocyte antigen 6 complex, locus C

UniProt

P0CW02

Host

Rat

Reactivity

Mouse

Immunogen

L3 cloned CTL cells

Target

Ly-6C

Isotype

IgG2c

Clone

HK1.4

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

0.5 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) recognizes an epitope on Mouse Ly-6C

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5

Aggregation

Aggregation level ≤ 5%

Applications Notes

Immunoprecipitation: 1–2 μg per 100–500 μg of total protein (1 ml of cell lysate) . Each investigator should determine their own optimal working dilution for specific applications.

Antibodies Against Same Target

Anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin [RB6-8C5] (ICH1131) Anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin [RB6-8C5] (ICH1131UL) Anti-Ly-6C In Vivo Antibody - Low Endotoxin [7B10] (ICH1095) Anti-Ly-6G In Vivo Antibody - Low Endotoxin [1A8] (ICH1089) Anti-Ly-6G In Vivo Antibody - Ultra Low Endotoxin [1A8] (ICH1089UL)