Anti-Mouse Dendritic Cells (33D1) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Dendritic cells (DC) are a small subpopulation of lymphoid cells with distinctive cytologic features, surface properties, and functions.

Product Name Alternative

DCIR2

Host

Rat

Reactivity

Mouse

Immunogen

Dendritic cells

Target

Dendritic Cells

Isotype

IgG2b

Clone

33D1

Applications

Western Blot, IHC (Frozen), Flow Cytometry, Immunocytochemistry

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

0.5 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles

Specificity

Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) recognizes Mouse Dendritic Cells

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

Clone 33D1 monoclonal antibody reacts with mouse dendritic cell (DC), specific surface marker, and DCIR2. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.