Anti-Mouse Ly-6G (1A8) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Ly-6G is a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Name Alternative

Lymphocyte antigen 6 complex, locus G

UniProt

P35461

Host

Rat

Reactivity

Mouse

Immunogen

Mouse Ly-6G transfected EL-4J cell line

Target

Ly-6G

Isotype

IgG2a

Clone

1A8

Applications

IHC (FFPE), IHC (Frozen), Flow Cytometry, Western Blot, In vivo Depletion, Immunfluorescence

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-Ly-6G In Vivo Antibody - Low Endotoxin (1A8) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-Ly-6G In Vivo Antibody - Ultra Low Endotoxin [1A8] (ICH1089)

Specificity

Anti-Ly-6G In Vivo Antibody - Low Endotoxin (1A8) recognizes an epitope on Mouse Ly-6G. Unlike clone RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Antibodies Against Same Target

Anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin [RB6-8C5] (ICH1131) Anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin [RB6-8C5] (ICH1131UL) Anti-Ly-6C In Vivo Antibody - Low Endotoxin [HK1.4] (ICH1111) Anti-Ly-6C In Vivo Antibody - Low Endotoxin [7B10] (ICH1095)