Anti-Mouse IL-4 (11B11) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.

Product Name Alternative

Interleukin-4, Il-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1

UniProt

P07750

Host

Rat

Reactivity

Mouse

Immunogen

Purified Recombinant Mouse IL-4 (>98%)

Target

IL-4

Isotype

IgG1 κ

Clone

11B11

Applications

ELISA Capture, ELISPOT, Flow Cytometry, Neutralization, Immunoprecipitation, Functional Assays, CyTOF, IHC

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

<0.5 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) is stable when stored at 2-8°C for 1 week. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Aggregation

Aggregation level ≤ 5%

Applications Notes

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 μg/ml. ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 μg/ml is recommended. Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo. Each investigator should determine their own optimal working dilution for specific applications.