Anti-CTLA-4 (9H10) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system. CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells. CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment. Clone 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA4 binding, allowing for CD28 binding.

Product Name Alternative

Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

UniProt

P09793

Host

Syrian Hamster

Reactivity

Mouse

Immunogen

Mouse CTLA-4-human IgG1 fusion protein

Target

CTLA-4

Isotype

IgG

Clone

9H10

Applications

Western Blot, Blocking, ELISA, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

References & Citations

Rodriguez-Baena, Francisco Javier et al. Microglial reprogramming enhances antitumor immunity and immunotherapy response in melanoma brain metastases. Cancer Cell, Volume 43, Issue 3, 413 - 427.e9

Shipping Conditions

Blue ice

Storage Conditions

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (9H10) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CTLA-4 In Vivo Antibody - Ultra Low Endotoxin [9H10] (ICH1084UL)

Specificity

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (9H10) recognizes an epitope on Mouse CTLA-4

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Activated T cells

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Antibodies Against Same Target

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin [UC10-4F10-11] (ICH1085) Anti-CTLA-4 In Vivo Antibody - Low Endotoxin [9D9] (ICH1096) Anti-CTLA-4 In Vivo Antibody - Ultra Low Endotoxin [9D9] (ICH1096UL)