Anti-Mouse CD172a (P84) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Signal regulatory protein alpha (SIRPalpha), also known as CD172a, is a regulatory membrane type I glycoprotein from the SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.

Product Name Alternative

Sirpa, Sirp-alpha-1, SHP substrate 1, SHPS-1, Shps1, Bit, p84, MyD-1 antigen, Myd1, Ptpns1

UniProt

P97797

Host

Rat

Reactivity

Mouse

Immunogen

Mouse brain membrane protein

Target

CD172a

Isotype

IgG1 κ

Clone

P84

Applications

Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD172a In Vivo Antibody - Low Endotoxin (P84) is stable when stored at 2-8°C. Do not freeze.

Specificity

Anti-CD172a In Vivo Antibody - Low Endotoxin (P84) recognizes an epitope on Mouse CD172a

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Monocytes, macrophages, myeloid cells, neuronal tissue

Aggregation

Aggregation level ≤ 5%

Applications Notes

Blocking: Reported applications in literature include: blocking SIRPalpha interaction with CD47 & in vivo blocking of dendritic cell migration. Each investigator should determine their own optimal working dilution for specific applications.