Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.

Product Name Alternative

Intercellular adhesion molecule 1, Icam1, MALA-2, MyD10

UniProt

P13597

Host

Rat

Reactivity

Mouse

Immunogen

Mouse NS-1 cells

Target

CD54

Isotype

IgG2b κ

Clone

YN1/1.7.4

Applications

Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) recognizes an epitope on Mouse ICAM-1

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.