Anti-Mouse CD45 (I3/2.3) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA) . CD45 plays a key role in TCR and BCR signal transduction.

Product Name Alternative

Receptor-type tyrosine-protein phosphatase C, Ptprc, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, LCA

UniProt

P06800

Host

Rat

Reactivity

Mouse

Immunogen

Mouse lymphoma cell line

Target

CD45

Isotype

IgG2b

Clone

I3/2.3

Applications

Western Blot, Flow Cytometry, IHC (Frozen), IHC (Paraffin)

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) recognizes an epitope on Mouse CD45

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD45 antigen is present on all cells of hematopoietic origin, but not erythrocytes

Aggregation

Aggregation level ≤ 5%

Applications Notes

Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤0.125 μg per million cells in 100 μl volume. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

Antibodies Against Same Target

Rat anti Mouse/Human CD45R In Vivo Antibody - Low Endotoxin [RA3-6B2] (ICH1130) Rat anti Mouse/Human CD45R In Vivo Antibody - Ultra Low Endotoxin [RA3-6B2] (ICH1130UL)