Anti-CD11C (N418) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD11c is a 150 kD glycoprotein primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells.

Product Name Alternative

Integrin alpha-X, Itgax, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95

UniProt

Q9QXH4

Host

Armenian Hamster

Reactivity

Mouse

Immunogen

Mouse spleen dendritic cells

Target

CD11c

Isotype

IgG

Clone

N418

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunofluorescence, Immunocytochemistry, ELISA

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD11C In Vivo Antibody - Low Endotoxin (N418) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD11C In Vivo Antibody - Low Endotoxin (N418) recognizes an epitope on Mouse CD11C

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)

Antibodies Against Same Target

Anti-CD11c In Vivo Antibody - Low Endotoxin [3.9] (ICH1008)

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