Anti-Mouse CD11a (FD441.8) In Vivo Antibody - Low Endotoxin

Product Specifications

Product Name Alternative

LFA-1 alpha, LFA-1A, Integrin alpha-L, ITGAL, Ly-15

UniProt

P24063

Host

Rat

Reactivity

Mouse

Target

CD11a

Isotype

IgG2b

Clone

FD441.8

Applications

Western Blot, Functional Assays, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD11a In Vivo Antibody - Low Endotoxin (FD441.8) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD11a In Vivo Antibody - Low Endotoxin (FD441.8) recognizes an epitope on Mouse CD11a

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD11a LFA-1 antigen is present on >97% thymocytes, >95% blood lymphocytes, 79% bone marrow cells and certain lymphoma and macrophage-like cell lines.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Functional Assay: The antibody inhibits ConA-facilitated lysis, but does not affect PHA-facilitated lysis. Each investigator should determine their own optimal working dilution for specific applications.