Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.

Product Name Alternative

Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3

UniProt

P10300

Host

Rat

Reactivity

Mouse

Immunogen

Mouse thymus or spleen

Target

CD8 beta

Isotype

IgG1

Clone

53-5.8

Applications

Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin [53-5.8] (ICH1046UL)

Specificity

Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1