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Anti-Mouse CD8a (2.43) In Vivo Antibody - Low Endotoxin

Product Specifications

Product Name Alternative

CD8 alpha chain, Lyt-2, Lyt2

UniProt

P01731

Host

Rat

Reactivity

Mouse

Target

CD8a

Isotype

IgG2b

Clone

2.43

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, Immunofluorescence, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

References & Citations

Tang, Dongyang et al. Augment proteasome inhibitor efficacy activates CD8+ T cell-mediated antitumor immunity in breast cancer. Cell Reports Medicine, Volume 6, Issue 7, 102211

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8a In Vivo Antibody - Low Endotoxin (2.43) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin [2.43] (ICH1045UL)

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/Anti-CD8a-In-Vivo-Antibody-Low-Endotoxin-2.43-ICH1045/

Specificity

Anti-CD8a In Vivo Antibody - Low Endotoxin (2.43) recognizes an epitope on Mouse CD8a

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

CD8a (Ly 2.2) is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

Antibodies Against Same Target

Anti-CD8a In Vivo Antibody - Low Endotoxin [53-6.7] (ICH1044) Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin [53-6.7] (ICH1044UL)

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