Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A (CD8a) /β (CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells.

Product Name Alternative

CD8 alpha chain, Lyt-2, Lyt2

UniProt

P01731

Host

Rat

Reactivity

Mouse

Immunogen

Mouse thymus or spleen

Target

CD8a

Isotype

IgG2a κ

Clone

53-6.7

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin [53-6.7] (ICH1044UL)

Specificity

Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins. Each investigator should determine their own optimal working dilution for specific applications.