Anti-Mouse CD8 (YTS-169) In Vivo Antibody - Low Endotoxin

Product Specifications

Host

Rat

Reactivity

Mouse

Immunogen

CBA mouse thymocytes

Target

CD8

Isotype

IgG2b

Clone

YTS-169

Applications

IHC (Frozen), In vivo Depletion, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

<0.5 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

References & Citations

Cao et al. Injectable hydrogel loaded with lysed OK-432 and doxorubicin for residual liver cancer after incomplete radiofrequency ablation. Journal of Nanobiotechnology

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8 In Vivo Antibody - Low Endotoxin (YTS-169) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CD8 In Vivo Antibody - Ultra Low Endotoxin [YTS-169] (ICH1043UL)

Specificity

Anti-CD8 In Vivo Antibody - Low Endotoxin (YTS-169) recognizes an epitope on Mouse CD8

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections but the concentration is assay dependent. Each investigator should determine their own optimal working dilution for specific applications.