Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.

Product Name Alternative

T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e

UniProt

P22646

Host

Syrian Hamster

Reactivity

Mouse

Immunogen

Mouse T-cell receptor

Target

CD3e

Isotype

IgG2a κ

Clone

500A2

Applications

Functional Assays, Flow Cytometry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [500A2] (ICH1040UL)

Specificity

Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Each investigator should determine their own optimal working dilution for specific applications.