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Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the Ig gene superfamily. CD86 is a ligand for CD28 and CD152 (CTLA-4) . CD86 is related to the CD80 molecule and functions as a counterreceptor for CD28 and CTLA-4. The GL1 clone has been shown to provide critical signals for T-Cell-dependent responses in vivo and in vitro.

Product Name Alternative

Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1

UniProt

P42082

Host

Rat

Reactivity

Mouse

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Target

CD86

Isotype

IgG2a κ

Clone

GL1

Applications

Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunocytochemistry, Blocking, Immunofluorescence, Western Blot, CyTOF

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) recognizes an epitope on Mouse CD86

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Frequently Asked Questions

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