Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

CD80 (B7-1) is a type I integral membrane protein related very closely to CD86 (B7-2) . A main function of CD80 is the costimulation of T-cells through ligation of CD28 and CD152 (CTLA-4) . T-cells activate with binding of CD28 to CD80 and binding of CD80 to CTLA-4 results in inhibition of T-cells. The clone 16-10A1 has the ability to block the costimulatory activity of CD80 while exhibiting no effect on the activity of CD86.

Product Name Alternative

Activation B7-1 antigen, B7

UniProt

Q00609

Host

Armenian Hamster

Reactivity

Mouse

Immunogen

Purified Recombinant Mouse CD80 (>98%)

Target

CD80

Isotype

IgG

Clone

16-10A1

Applications

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Related Products

Anti-CD80 In Vivo Antibody - Ultra Low Endotoxin [16-10A1] (ICH1037UL)

Specificity

Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) recognizes an epitope on Mouse CD80

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)

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