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Bulk Anti-Human iNKT Cell (6B11) Antibody

Product Specifications

Background

Encoded by the TCR V alpha 24-J alpha 18 germline configuration, V alpha 24-J alpha Q is expressed on a subset of NKT cells, namely invariant NKT (iNKT) . V alpha 24-J alpha Q TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production.

Product Name Alternative

TCR Valpha24-Jalpha18, TCR Valpha24-JalphaQ, invariant NKT cell, iNKT

Host

Mouse

Reactivity

Human

Immunogen

Unknown

Target

INKT cell

Isotype

IgG1 κ

Clone

6B11

Applications

Western Blot, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-iNKT cell In Vivo Antibody - Low Endotoxin (6B11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-iNKT cell In Vivo Antibody - Low Endotoxin (6B11) recognizes an epitope on TCR V alpha 24-J alpha 18

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Invariant NKT cells

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)

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