Bulk Anti-Human HLA-DR (L243) Antibody

Product Specifications

Background

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD (heavy) chain & a 27 kD (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, and dendritic cells. HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Clone L243 binds a conformational epitope on HLA-DRalpha which depends on the correct folding of the Aβ heterodimer.

Product Name Alternative

Major Histocompatibility Class II, MHC class II, HLA-DR Monomorphic

Host

Mouse

Reactivity

Human

Immunogen

Unknown

Target

HLA-DR

Isotype

IgG2a

Clone

L243

Applications

Western Blot, Blocking, Flow Cytometry, Immunoprecipitation, CyTOF, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

References & Citations

Yuan et al. WNT4 promotes macrophage polarization via granulosa cell M-CSF and reduces granulosa cell apoptosis in endometriosis

Shipping Conditions

Blue ice

Storage Conditions

Anti-HLA-DR In Vivo Antibody - Low Endotoxin (L243) is stable for at least four (4) weeks when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-HLA-DR In Vivo Antibody - Low Endotoxin (L243) recognizes/reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross-react with HLA-DP and HLA-DQ

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4.

Antigen Distribution

The HLA-DR antigen is present on B-lymphocytes, activated T-lymphocytes and activated natural killer (NK) lymphocytes. This antigen is also expressed on monocytes, macrophages, human progenitor cells and B-cell lymphomas.