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Bulk Anti-Human HLA-DQ (1a3) Antibody

Product Specifications

Product Name Alternative

HLA-DQ Monomorphic

Host

Mouse

Reactivity

Human

Target

HLA-DQ

Isotype

IgG2a

Clone

1a3

Applications

Western Blot, ELISA

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-HLA-DQ In Vivo Antibody - Low Endotoxin (1a3) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-HLA-DQ In Vivo Antibody - Low Endotoxin (1a3) recognizes a monomorphic epitope present on HLA-DQ molecules of the human class II major histocompatibility complex (MHC) . anti-HLA-DQ does not react with HLA-DR or HLA-DP molecules.

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4.

Antigen Distribution

The HLA-DQ antigen is present on approximately 10% of peripheral blood lymphocytes. This antibody reacts with virtually all B-cell lines and some tissue macrophages and dendritic cells.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)

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