Bulk Anti-Human HLA-DP (B7/21) Antibody

Product Specifications

Product Name Alternative

HLA-DP Monomorphic

Host

Mouse

Reactivity

Human

Immunogen

Unknown

Target

HLA-DP

Isotype

IgG3

Clone

B7/21

Applications

Western Blot, Immunocytochemistry, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

References & Citations

Saggau, Carina et al. Autoantigen-specific CD4+ T cells acquire an exhausted phenotype and persist in human antigen-specific autoimmune diseases. Immunity, Volume 57, Issue 10, 2416 - 2432.e8

Shipping Conditions

Blue ice

Storage Conditions

Anti-HLA-DP In Vivo Antibody - Low Endotoxin (B7/21) is stable for at least six (6) weeks when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-HLA-DP In Vivo Antibody - Low Endotoxin (B7/21) recognizes a (Mr 26-34 kDa) class II major histocompatibility complex (MHC) antigen. The antibody recognizes a monomorphic determinant present on cells expressing DP1, DP2, DP3, DP4 and DP5.

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4.

Antigen Distribution

The HLA-DP antigen is present on approximately 10% of normal peripheral blood lymphocytes but not on resting peripheral T-lymphocytes. HLA-DP is present in low density on peripheral blood monocytes and mitogen-stimulated T-lymphoblasts. Anti-HLA-DP reacts with most B-cell lines.