Bulk Anti-Human CD11c (3.9) Antibody

Product Specifications

Background

Anti-Human CD11c may be used to stain monocytes and macrophages or hairy cell leukemia cells. This antibody can be used in studies of complement receptors. In malignant tissue, Anti-CD11c stains true histiocytic lymphomas and does not stain carcinomas or T- or B-lymphomas.

Product Name Alternative

Integrin alpha-X, ITGAX, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95

UniProt

P20702

Host

Mouse

Reactivity

Human

Target

CD11c

Isotype

IgG1

Clone

3.9

Applications

Flow Cytometry, CyTOF, IHC (Frozen), Immunofluorescence

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD11c In Vivo Antibody - Low Endotoxin (3.9) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD11c In Vivo Antibody - Low Endotoxin (3.9) recognizes Human CD11c. Anti-Human CD11c the (Mr 150 kDa) a-chain of the CD11c/CD18 complex. The antigen is the complement receptor type 4 (CR4) . CD11c is an a/B heterodimeric glycoprotein which belongs to the B2 integrin family. It is also known as gp 150/95.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD11c antigen is present on monocytes and in lower density on granulocytes and NK cells. The antigen is strongly expressed in hairy cell leukemia and in some acute myeloid leukemias. Anti-Human CD11c (clone 3.9) stains macrophages in acetone-fixed frozen section of human lung, brain, colon, skin, spleen, thymus and tonsil.