Bulk Anti-Human CD11b (ICRF44) Antibody

Product Specifications

Background

Anti-CD11b may be used to study lymphocyte subsets in flow cytometry and in cell adhesion research (Mac-1) . The antibody may be used to stain tissue sections which have been acetone fixed and frozen.

Product Name Alternative

Integrin alpha-M, ITGAM, ICR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, CR3A

UniProt

P11215

Host

Mouse

Reactivity

Human

Immunogen

Unknown

Target

CD11b

Isotype

IgG1

Clone

ICRF44

Applications

Flow Cytometry, Western Blot, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and analyzed by silver stain.

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD11b In Vivo Antibody - Low Endotoxin (44) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD11b In Vivo Antibody - Low Endotoxin (44) recognizes Human CD11b. Anti-Human CD11b recognizes the alpha subunit of the CD11b/CD18 complex, an alpha/Beta heterodimeric surface glycoprotein on human leukocytes (Mr 165-170 kDa) . The CD11b/CD18 antigen belongs to the B2 integrin family. Other names include Mac-1, Cr3, MO-1 and C3bi receptor. The CD11b molecule functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD11b antigen is expressed on approximately 30% of human peripheral blood lymphocytes, most natural killer lymphocytes, circulating monocytes, granulocytes, a subset of T-cells and subsets of macrophages.