Anti-Phospho-IRF3 (S396) Antibody

IRF3 (interferon regulatory factor 3) ?is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with?CREBBP. IRF3 plays an important role in the?innate immune system's response to?viral infection. Aggregated?MAVS?have been found to activate IRF3 dimerization. Although?IRF3?increased transcriptional activity from an ISRE-containing promoter, expression of?IRF3?as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites.?Translocation of?IRF3?was accompanied by an increase in serine and threonine phosphorylation.?The transcriptional activators CREBBP and EP300 coimmunoprecipitated with?IRF3?only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.