Human recombinant R-spondin 1 protein, AF
R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.
Product Specifications
Synonyms
RSPO1, Roof plate-specific spondin-1
Gene Name
RSPO1
UniProt
Q2MKA7
Reactivity
Human
Tag
His-SUMO Tag (N-term)
Source
HEK293 cell
Applications
Cell Culture
Purification
>95% as determined by SDS-PAGE.
Endotoxin
<1 EU per 1 μg of the protein by the LAL method.
Bioactivity
R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED50 for this effect is 1-3 μg/mL.
Form
Liquid (1X PBS, pH 7.4) If you have any concerns or special requirements, please confirm with us.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Molecular Weight
The protein has a calculated MW of 46.8 kDa. The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis) .
Storage Conditions
AA Sequence
A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus.
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