Human recombinant IGFBP7 (IGF-binding protein 7) protein, AF

IGFBP7, also known as mac25, prostacyclin-stimulating factor (PSF), tumor adhesion factor (TAF), and angiomodulin (AGM), is a secreted protein that belongs to the insulin-like growth factor (IGF) -binding protein (IGFBP) family. In contrast to the other family members, IGFBP7 binds IGFs through the N-terminal domain with low affinity. IGFBP7 is expressed in various human tissues, including the brain, liver, pancreas, and skeletal muscle, and is secreted into circulation. IGF-BP7 modulates many biological functions, such as protein synthesis, proliferation, anti-apoptosis, and cell survival. Moreover, it has been reported that IGFBP7 promotes cardiac senescence through IGF-1R/IRS/AKT-dependent suppression of FOXO3a, inhibiting DNA repair, subsequently the leading to the progression of Heart failure. Human and mouse IGFBP7 are highly homologous, sharing 94% a.a. sequence identity.

Product Specifications

Synonyms

IBP-7, IGFBP-7, IGFBP-rP1, MAC25, PSF (Prostacyclin-stimulating factor), TAF (Tumor-derived adhesion factor)

Gene Name

IGFBP7

UniProt

Q16270

Reactivity

Human

Tag

His-SUMO Tag (N-term)

Source

HEK293 cell

Applications

Cell Culture

Purification

>95% as determined by SDS-PAGE.

Endotoxin

<1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Testing in process

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 38.18 kDa. The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.