Human recombinant GFAP protein, AF

GFAP (Glial Fibrillary Acidic Protein) is a protein primarily found in CNS astrocytes. Increased GFAP immunoreactivity indicates gliosis, a response to neural damage. GFAP defects cause Alexander disease, a rare CNS disorder with astrocytic Rosenthal fiber accumulation. The infantile form leads to myelination failure and early mortality, while the juvenile or adult forms present with ataxia, bulbar signs, spasticity, progressing more gradually.

Product Specifications

Synonyms

Glial fibrillary acidic protein

Gene Name

GFAP

UniProt

P14136

Reactivity

Human

Tag

His Tag (C-term)

Source

Escherichia coli

Applications

Cell Culture

Purification

>98% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Bioactivity

Testing in process

Form

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Molecular Weight

The protein has a calculated MW of 51 kDa. The protein migrates as 50-55 kDa under reducing condition (SDS-PAGE analysis) .

Storage Conditions

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.