Human recombinant IL-1 beta (Interleukin-1 beta) protein, AF
Interleukin-1 beta (IL-1β) is a major cytokine expressed in macrophage, NK cells, monocytes, and neutrophils. It also plays fundamental role in inflammatory response, including monocyte activation, which is essential for the host defense and pathogen and pathogen resistance. Pro-IL-1β is cleaved by cytosolic caspase 1 to form mature IL-1β.
Product Specifications
Synonyms
Catabolin, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endoge_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x0002_nous Mediator (LEM), Mononuclear Cell Factor (MCF)
Gene Name
IL1B
UniProt
P01584
Reactivity
Human
Tag
His Tag (C-term)
Source
Escherichia coli
Applications
Cell Culture
Purification
>98% as determined by SDS-PAGE.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Bioactivity
Measure by its ability to induce IL-8 secretion in HT29 cells. The ED50 for this effect is <2.0 ng/mL. The specific activity of recombinant human IL-1 beta is approximately >3 x 107 IU/mg.
Form
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Molecular Weight
The protein has a calculated MW of 18.5 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis) .
Storage Conditions
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
AA Sequence
MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus.
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