Human UCH-L5/UCH37 Recombinant Protein
Human UCH-L5/UCH37 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-329aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, INO80 complex subunit R, INO80R, UCH37, CGI-70, AD-019
Gene Name
UCHL5
UniProt
Q9Y5K5
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Ubiquitination
Purification
> 90% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 500 pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
39.7 kDa (349aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA
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