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Mouse Ak1 Recombinant Protein

Mouse Ak1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-210aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Adenylate kinase isoenzyme 1, Adenylate kinase 1, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase 1, Myokinase

Gene Name

Ak1

UniProt

Q9R0Y5

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Metabolism

Purification

> 90% by SDS-PAGE

Concentration

0.5mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

25.5 kDa (233aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

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