Mouse Ak1 Recombinant Protein
Mouse Ak1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-210aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Adenylate kinase isoenzyme 1, Adenylate kinase 1, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase 1, Myokinase
Gene Name
Ak1
UniProt
Q9R0Y5
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Metabolism
Purification
> 90% by SDS-PAGE
Concentration
0.5mg/mL (determined by absorbance at 280nm)
Bioactivity
Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
25.5 kDa (233aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
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