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Mouse Uchl3 Recombinant Protein

Mouse Uchl3 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 1-230aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin thioesterase L3, UCH-L3

Gene Name

Uchl3

UniProt

Q9JKB1

Tag

His-Tag

Source

Baculovirus

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Ubiquitination

Purification

> 90% by SDS-PAGE

Concentration

0.5mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

27.2 kDa (238aa) 28-40kDa (SDS-PAGE under reducing conditions)

Contents

Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

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