Mouse ALT1/GPT1 Recombinant Protein
Mouse ALT1/GPT1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-496aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Alanine aminotransferase 1, ALT, ALT1, Gpt-1, Gpt1
Gene Name
Gpt
UniProt
Q8QZR5
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Metabolism
Purification
> 90% by SDS-PAGE
Concentration
0.5mg/mL (determined by absorbance at 280nm)
Bioactivity
Specific activity is > 100 units/mg, and is defined as the amount of enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
57.5 kDa (519aa) confirmed by MALDI-TOF
Contents
Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 10% glycerol
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