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Mouse ALT2/GPT2 Recombinant Protein

Mouse ALT2/GPT2 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-522aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Alanine aminotransferase 2, ALT2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, Glutamate pyruvate transaminase 2

Gene Name

Gpt2

UniProt

Q8BGT5

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Metabolism

Purification

> 85% by SDS-PAGE

Concentration

1mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

60.1 kDa (542aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 2mM DTT

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