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Mouse 5'-Nucleotidase/CD73 Recombinant Protein

Mouse 5'-Nucleotidase/CD73 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 29-551aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

CD73, 5-NT, 5'-nucleotidase, 5'-nucleotidase ecto, AI447961, E5NT, ecto, eN, eNT, NT, NT5, Nt5e, NTE, 2210401F01Rik

Gene Name

Nt5e

UniProt

Q61503

Tag

His-Tag

Source

Baculovirus

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Cardiovascular

Purification

> 90% by SDS-PAGE

Concentration

0.5mg/mL (determined by absorbance at 280nm)

Bioactivity

Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

59.1 kDa (531aa)

Contents

Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

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