Human Thioredoxin Reductase 1/TXNRD1 Recombinant Protein
Human Thioredoxin Reductase 1/TXNRD1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 161-647aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
GRIM 12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, TR, TR 1, TRXR 1, TXNR, TXNRD 1
Gene Name
TXNRD1
UniProt
Q16881
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Redox Proteins
Purification
> 95% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
55.7 kDa (508aa) confirmed by MALDI-TOF
Contents
Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
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