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Human Thioredoxin Reductase 1/TXNRD1 Recombinant Protein

Human Thioredoxin Reductase 1/TXNRD1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 161-647aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

GRIM 12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, TR, TR 1, TRXR 1, TXNR, TXNRD 1

Gene Name

TXNRD1

UniProt

Q16881

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Redox Proteins

Purification

> 95% by SDS-PAGE

Concentration

0.5mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

55.7 kDa (508aa) confirmed by MALDI-TOF

Contents

Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

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