Human Glyoxalase II Recombinant Protein
Human Glyoxalase II Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-260aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
Gene Name
HAGH
UniProt
Q16775
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Enzymes and Proteases
Purification
> 95% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 500 units/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
31.4 kDa (284aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
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