Human BLMH/Bleomycin Hydrolase Recombinant Protein
Human BLMH/Bleomycin Hydrolase Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-455aa. Application (s) : SDS-PAGE, Enzyme Activity.
Product Specifications
Synonyms
Bleomycin hydrolase, BH, BLM hydrolase, BMH
Gene Name
BLMH
UniProt
Q13867
Tag
His-Tag
Source
E. coli
Applications
SDS-PAGE, Enzyme Activity
Field of Research
Neuroscience
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1 pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
54.7 kDa (475aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
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