Human Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein
Human Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-323aa. Application (s) : Enzyme Activity, SDS-PAGE.
Product Specifications
Synonyms
Aldo-keto reductase family 1 member C1, DDH1, DDH, MBAB, DD1, HAKRC, Dihydrodiol dehydrogenase 1, 20-alpha (3-alpha) -hydroxysteroid dehydrogenase, High-affinity hepatic bile acid-binding protein (HBAB)
Gene Name
AKR1C1
UniProt
Q04828
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Redox Proteins
Purification
> 90% by SDS-PAGE
Concentration
0.5mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
38.9 kDa (343aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol
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