Human Glyoxalase I Recombinant Protein
Human Glyoxalase I Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-184aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Product Specifications
Synonyms
Lactoylglutathione lyase, Glyoxalase I, Glx 1, GLO-1 Methylglyoxalase, Aldoketomutase
Gene Name
GLO1
UniProt
Q04760
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Enzymes and Proteases
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
20.7 kDa (184aa) confirmed by MALDI-TOF
Contents
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
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